Curcumin Stimulates Proliferation of Spinal Cord Neural Progenitor Cells via a Mitogen-Activated Protein Kinase Signaling Pathway
Journal of Korean Neurosurgical Society 2014³â 56±Ç 1È£ p.1 ~ p.4
(Son Si-Hoon) - Kyungpook National University Hospital Department of Neurosurgery
±è°æÅÂ(Kim Kyoung-Tae) - Kyungpook National University Hospital Department of Neurosurgery
Á¶´ëö(Cho Dae-Chul) - Kyungpook National University Hospital Department of Neurosurgery
±èÇýÁ¤(Kim Hye-Jeong) - Kyungpook National University Hospital Department of Neurosurgery
¼ºÁÖ°æ(Sung Joo-Kyung) - Kyungpook National University Hospital Department of Neurosurgery
¹èÀ缺(Bae Jae-Sung) - Kyungpook National University School of Medicine Department of Physiology
Abstract
Objective: The aims of our study are to evaluate the effect of curcumin on spinal cord neural progenitor cell (SC-NPC) proliferation and to clarify the mechanisms of mitogen-activated protein (MAP) kinase signaling pathways in SC-NPCs.
Methods: We established cultures of SC-NPCs, extracted from the spinal cord of Sprague-Dawley rats weighing 250 g to 350 g. We measured proliferation rates of SC-NPCs after curcumin treatment at different dosage. The immuno-blotting method was used to evaluate the MAP kinase signaling protein that contains extracellular signal-regulated kinases (ERKs), p38, c-Jun NH2-terminal kinases (JNKs) and ¥â-actin as the control group.
Results: Curcumin has a biphasic effect on SC-NPC proliferation. Lower dosage (0.1, 0.5, 1 ¥ìM) of curcumin increased SC-NPC proliferation. However, higher dosage decreased SC-NPC proliferation. Also, curcumin stimulates proliferation of SC-NPCs via the MAP kinase signaling pathway, especially involving the p-ERK and p-38 protein. The p-ERK protein and p38 protein levels varied depending on curcumin dosage (0.5 and 1 ¥ìM, p<0.05).
Conclusion: Curcumin can stimulate proliferation of SC-NPCs via ERKs and the p38 signaling pathway in low concentrations.
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Curcumin, Spinal cord neural progenitor cell, Mitogen activated protein kinase
KMID :
1001920140560010001
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